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Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
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JoVE Journal Medicina
Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

DOI:
10.3791/63293-v

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07:34 min

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December 16, 2021

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, ,
1Department of Microbiology and Immunology,University of Texas Medical Branch

Capítulos

  • 00:04Introduction
  • 00:49Isolation and Transfer of Splenic CBir1 TCR Tg CD4+ T Cells
  • 04:39Colon Collection and Histopathological Scoring
  • 06:03Results: Morphological and Histopathological Features of the Adoptive Transfer Colitis Model
  • 07:11Conclusion

Summary

Tadução automática

Tadução automática

In this protocol, a gut microbiota antigen-specific T cell adoptive transfer colitis model is described. CD4+ T cells are isolated from CBir1 TCR transgenic mice. These are specific for an immunodominant gut microbiota antigen CBir1 flagellin, which is transferred into recipient Rag1-/- mice, leading to intestinal inflammation.

Tags

Inflammatory Bowel DiseaseIBDColitis ModelCbir FlagellinT-cell ResponsesAdoptive TransferCD4+ T CellsImmunodeficient MiceCell IsolationMagnetic SeparationCell Counting

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