/
/
Cultivo de linfocitos en microgravedad simulada utilizando un sistema de cultivo celular rotativo
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine,University of Toronto, 2Krembil Research Institute, Toronto Western Hospital,University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital,Sinai Health Systems, 4Medical Oncology and Hematology,Princess Margaret Cancer Centre
Capítulos
- 00:04Introduction
- 00:48Preparation of Vessels for Cell Culture
- 01:43Loading Vessels with Stock Cell Culture
- 04:42Removing Bubbles from the Vessels
- 05:47Attaching the Vessel to the Rotating Base
- 06:36Treatment and Harvesting Cells from the Vessels
- 08:06Results: Application of the Rotary Cell Culture System to Study the Effects of Simulated Microgravity on Lymphocytes
- 08:55Conclusion
Esta es una guía paso a paso para usar un sistema de cultivo celular rotativo disponible comercialmente para cultivar linfocitos en microgravedad simulada utilizando recipientes de cultivo desechables especializados. Este método de cultivo se puede aplicar a cualquier cultivo celular de tipo suspensión.
Tags
Keywords:
LymphocytesSimulated MicrogravityRotary Cell Culture SystemImmune CellsSpace EnvironmentHuman PhysiologyMolecular LevelEconomically FeasibleCell CultureControlTreatmentComplete MediaIncubatorFlask ControlT25 Suspension Culture FlaskStock Cell CultureSyringe Port CapsStopcocksFill PortSerological PipetteVacuum System
