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Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms
DOI:

05:50 min

January 20, 2022

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Capítulos

  • 00:04Introduction
  • 00:47Preparation of Stock Solutions and Gelification Mixture
  • 02:53Preparation of qPCR Master Mix, Gelification of the Reagents in the Reaction Vessels, and Gelified qPCR
  • 04:22Results: Ready to Use Gelified qPCR for Trypanosoma cruzi Epimastigotes DNA Detection
  • 05:11Conclusion

Summary

Tadução automática

The present work describes the steps for producing ready-to-use qPCR for T. cruzi DNA detection that can be pre-loaded on the reaction vessel and stored in the refrigerator for several months.

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