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Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
 

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Article DOI: 10.3791/63322-v • 09:22 min • May 31st, 2022
May 31st, 2022 •
*1, *1, 1,2
1Department of Chemistry, University of Wisconsin-Madison, 2School of Pharmacy, University of Wisconsin-Madison
* These authors contributed equally

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Mass spectrometric characterization of neuropeptides provides sequence, quantitation, and localization information. This optimized workflow is not only useful for neuropeptide studies, but also other endogenous peptides. The protocols provided here describe sample preparation, MS acquisition, MS analysis, and database generation of neuropeptides using LC-ESI-MS, MALDI-MS spotting, and MALDI-MS imaging.

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Keywords: Neuropeptides Mass Spectrometry Callinectes Sapidus Crustacean Tissue Extraction Sample Preparation Desalting Quantitation Localization Biomarkers
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