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使用带有可更换盖子的乳腺成像窗口进行纵向活体腔内显微镜检查

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

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使用带有可更换盖子的乳腺成像窗口进行纵向活体腔内显微镜检查

DOI:

10.3791/63326-v

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10:53 min

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January 20, 2022

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Larissa Mourao², Marta Ciwinska², Jacco van Rheenen, Colinda L. G. J. Scheele²

¹Center for Cancer Biology,VIB (BE), ²Department of Oncology,KU Leuven (BE), ³Netherlands Cancer Institute, Department of Molecular Pathology,Oncode Institute (NL)

Capítulos

00:05Introduction
01:03Preparation of the R.MIW
02:13R.MIW Implantation
05:19Repeated Intravital Microscopy Through the R.MIW
07:03Opening and Closing the Lid of the R.MIW in Between Imaging Sessions
07:52Results: Multi-Day IVM of Mammary Gland Remodeling
10:11Conclusion

Summary

Tadução automática

该协议描述了一种带有可更换盖子（R.MIW）的新型乳腺成像窗口。植入R.MIW后的活体显微镜检查允许在不同发育阶段以细胞分辨率对健康和患病的乳腺进行纵向和多日成像。

Tags

Keywords: Mammary Imaging Window Longitudinal Intravital Microscopy Mammary Gland Mammary Tumor Single-cell Imaging Cyanoacrylate Adhesive Glass Cover Slip Surgical Procedure Region-of-interest

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