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Capítulos
- 00:04Introduction
- 00:55Preparation of Buffer, Tissue Sample, and Solution
- 02:15Total Protein Extraction
- 03:42SDS-PAGE and Western Blot Analysis
- 04:39Ammonium Sulfate Precipitation
- 06:28Ionic Exchange Chromatography with FPLC
- 08:14Protein Extraction Using Defined Sub-Protocols for Ammonium Sulfate-Precipitation and FPLC
- 09:32Results: Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
- 11:24Conclusion
Dieses Protokoll beschreibt, wie das domänenhaltige Fumarylacetoacetat-Hydrolase-haltige Protein 1 (FAHD1) aus Schweineniere und Mausleber extrahiert werden kann. Die aufgeführten Methoden können an andere Proteine von Interesse angepasst und für andere Gewebe modifiziert werden.
