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細胞外フラックス解析を用いた単一3D微細組織スフェロイドのミトコンドリアエネルギー代謝の探索
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JoVE Journal Pesquisa do Câncer
Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis
DOI:

08:15 min

February 03, 2022

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Capítulos

  • 00:05Introduction
  • 01:03Probing Mitochondrial Energy Metabolism of Single Spheroids Using XF Technology
  • 02:44Preparation of XF Assay Medium
  • 03:08Preparation of the Sensor Cartridge
  • 04:25Seeding Single Spheroids into the Spheroid Assay Microplate
  • 05:04Preparation and Loading of Compounds into the Sensor Cartridge for XF Assays
  • 05:31Results: Mitochondrial Energy Metabolism in 3D Cancer Cell-Line-Derived Spheroids
  • 07:35Conclusion

Summary

Tadução automática

これらのプロトコルは、タツノオトシゴの細胞外フラックス分析を使用して、3Dがん細胞株由来スフェロイドにおけるミトコンドリアエネルギー代謝を調べるのに役立ちます。

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