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Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model
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Neurociência
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JoVE Journal Neurociência
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

DOI:
10.3791/63355-v

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07:32 min

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December 28, 2021

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, , , , , ,
1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy,Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine,Universiti Malaya

Capítulos

  • 00:05Introduction
  • 01:00Zebrafish Maintenance and Pre-ICV Microinjection Preparations
  • 02:19Anaesthetisation and ICV Injection of Zebrafish
  • 04:10Locomotor Assessment
  • 05:22Results: Assessment of Neuroregeneration in Zebrafish Based PD Model
  • 06:44Conclusion

Summary

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The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.

Tags

Keywords: ZebrafishParkinson’s Disease6-hydroxydopamineLocomotor AssessmentMotor ImpairmentDopaminergic NeuronsIntracerebroventricular InjectionNeuroregenerationCell ReplacementTricaine MethanesulfonateAnesthesiaMicrocapillary Injector

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