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Recording Gap Junction Current from Xenopus Oocytes
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Biologia
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JoVE Journal Biologia
Recording Gap Junction Current from Xenopus Oocytes

Recording Gap Junction Current from Xenopus Oocytes

DOI:
10.3791/63361-v

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09:04 min

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January 21, 2022

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,
1Department of Neuroscience,University of Connecticut School of Medicine

Capítulos

  • 00:04Introduction
  • 00:42Preparation of Defolliculated Oocytes
  • 01:27GJ Protein Expression
  • 03:03Testing the Acquisition System with the Model Cell Supplied with the Amplifier
  • 04:09Set Up the Recording Stage and the Reference Electrode
  • 04:48Set Up the VDIFF Electrodes
  • 05:51Set Up the Current, Voltage Electrodes, Data Acquisition
  • 06:57Results: Representative Recording Traces
  • 08:05Conclusion

Summary

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Tadução automática

Here we present a protocol to express gap junction proteins in Xenopus oocytes and record junctional current between two apposed oocytes using a commercial amplifier designed for dual oocyte voltage-clamp recordings in a high side current measuring mode.

Tags

Keywords: Xenopus OocytesGap JunctionConnexinsInnexinsBiophysical PropertiesVoltage ClampOocyte InjectionCRNAOocyte PairingModel CellDifferential Voltage ProbeCurrent Cables

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