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Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

DOI:
10.3791/63374-v

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08:01 min

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April 21, 2022

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, ,
1Department of Translational Molecular Pathology,University of Texas MD Anderson Cancer Center

Capítulos

  • 00:05Introduction
  • 00:45Digestion of Tumor Tissue
  • 03:16Generation of Primary Tumor Cell Line
  • 04:47Characterization and Banking of Established Primary Tumor Cell Line
  • 06:13Results: Determination of Fibroblast Contamination of Cell Culture and Flow Cytometry Surface Staining of Cell Lines
  • 07:12Conclusion

Summary

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The goal of this method paper is to demonstrate a robust and reproducible methodology for the enrichment, generation, and expansion of primary tumor cell lines from surgically resected pleural mesothelioma.

Tags

Primary Malignant Pleural MesotheliomaTumor Cell ExpansionIn Vitro ModelTumor Digesting Enzymatic CocktailTumor DissociationCell StrainerTumor Digestion MediumCell CultureFibroblast Contamination

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