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Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
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Biologia
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JoVE Journal Biologia
Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

DOI:
10.3791/63396-v

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10:33 min

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May 27, 2022

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, ,
1Department of Biology,McMaster University

Capítulos

  • 00:04Introduction
  • 00:50Incubation of Soil in Liquid Broth and Transferring the Supernatant to the Solid Medium
  • 02:34Detection of Yeasts, Streaking for Single Colonies, and Identification of Yeast Species
  • 04:04Isolation of Aspergillus fumigatus from Soil
  • 07:32Results: Isolation and Characterization of Soil Yeast and Aspergillus fumigatus Populations
  • 09:37Conclusion

Summary

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This protocol is an effective, speedy method of culturing yeasts and the mold Aspergillus fumigatus from large sets of soil samples in as little as 7 days. The methods can be easily modified to accommodate a range of incubation media and temperatures as needed for experiments.

Tags

Keywords: Soil SamplesYeast DiversityMold DiversityFungal PopulationCulturable YeastsCulturable MoldsChloramphenicolBenomylYeast Extract-peptone-dextrose AgarRoller Drum IncubationBiosafety CabinetSoil Particle SuspensionColony SelectionStreak Plating

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