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Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex
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Neurociência
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JoVE Journal Neurociência
Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex

Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex

DOI:
10.3791/63404-v

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04:58 min

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December 03, 2021

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,
1Department of Science Education,Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, 2Department of Psychology,Sacred Heart University

Capítulos

  • 00:04Introduction
  • 00:48Sectioning of Brain Tissues and Mounting
  • 02:04Staining and Dehydration of Brain Tissue
  • 03:06Determination of Dendritic Spine Density
  • 03:48Results: Observation of Hippocampus and Effect of Environmental Enrichment on CA1 and mPFC
  • 04:23Conclusion

Summary

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The protocol describes a modification of the rapid Golgi method, which can be adapted to any part of the nervous system, for staining neurons in the hippocampus and medial prefrontal cortex of the rat.

Tags

Keywords: Golgi StainDendritic SpineHippocampusPrefrontal CortexCryostat SectioningRapid LabelingGolgi ImpregnationNeuron AnalysisDendritic LengthSpine Density

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