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In vivo Beeldvorming van biologische weefsels met gecombineerde twee-fotonenfluorescentie en gestimuleerde Raman-verstrooiingsmicroscopie
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1Department of Electronic and Computer Engineering,The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience,The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health,The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center,The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences,Southern University of Science and Technology
Capítulos
- 00:04Introduction
- 00:59Starting-Up the Lasers
- 02:02Optical Alignment of the Combined TPEF-Stimulated Raman Scattering Microscope
- 04:39Optimizing Imaging Conditions
- 06:36In Vivo TPEF-SRS Imaging of Mouse Spinal Cord
- 08:03Results: In Vivo Dual-Modal Imaging of Spinal Axons and Myelin Sheaths
- 08:31Conclusion
Gestimuleerde Raman-verstrooiing (SRS) microscopie maakt labelvrije beeldvorming van biomoleculen mogelijk op basis van hun intrinsieke vibratie van specifieke chemische bindingen. In dit protocol wordt de instrumentele opstelling van een geïntegreerde SRS- en twee-fotonenfluorescentiemicroscoop beschreven om cellulaire structuren in het ruggenmerg van levende muizen te visualiseren.
