Culturing and Screening the Plant Parasitic Nematode <em>Ditylenchus dipsaci</em>

Savina R. Cammalleri; Jessica Knox; Peter J. Roy

doi:10.3791/63438

Journal

/

Biologia

/

植物寄生线虫的培养和筛选

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

Please note that all translations are automatically generated. Click here for the English version.

植物寄生线虫的培养和筛选

DOI:

10.3791/63438-v

•

08:04 min

•

January 31, 2022

•

Savina R. Cammalleri^2,3, Jessica Knox³, Peter J. Roy^3,4

¹Department of Molecular Genetics,University of Toronto, ²Department of Biochemistry,University of Toronto, ³The Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, ⁴Department of Pharmacology and Toxicology,University of Toronto

Capítulos

00:05Introduction
00:38Culturing of D. dipsaci
03:22Extraction and Collection of D. dipsaci
04:14In Vitro Small Molecule Screen
05:36Data Collection and Analysis
06:13Results: In Vitro Small Molecule Screen Assay for Candidate Nematicides
07:40Conclusion

Summary

Tadução automática

本方案描述了一种可靠而直接的培养、收集和筛选 Ditylenchus dipsaci的方法。

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