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Biologia
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Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna
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Biologia
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JoVE Journal Biologia
Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna

Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna

DOI:
10.3791/63470-v

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11:47 min

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February 01, 2022

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, , , , , , , ,
1Botanical Institute,Karlsruhe Institute of Technology KIT, 2Institute for Biological Interfaces 5 (IBG 5),Karlsruhe Institute of Technology KIT, 3Institut für Technische Mikrobiologie,Hochschule Mannheim

Capítulos

  • 00:05Introduction
  • 01:05Natural Transformation and GFP Expression
  • 04:06Motility of Phormidium lacuna
  • 05:34Assay for Movement on the Surface
  • 06:27Assay for Phototaxis
  • 08:32Results: Natural Transformation: Integration and Segregation of Insert, Vector for sfGFP, sfGFP Expression, and Motility Studies
  • 11:01Conclusion

Summary

Tadução automática

Tadução automática

Phormidium lacuna is a filamentous cyanobacterium that was isolated from marine rockpools. This article describes the isolation of filaments from natural sources, DNA extraction, genome sequencing, natural transformation, expression of sfGFP, cryoconservation, and motility methods.

Tags

Natural TransformationFilamentous CyanobacteriumPhormidium LacunaOscillatorialesProtein ExpressionCryoconservationMotion StudiesHomogenizationCentrifugationKanamycin SelectionMicroscopyTransformed Filaments

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