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JoVE Journal Neuroscience
In vivo Positron Emission Tomography to Reveal Activity Patterns Induced by Deep Brain Stimulation in Rats
 

In vivo Positron Emission Tomography to Reveal Activity Patterns Induced by Deep Brain Stimulation in Rats

Article DOI: 10.3791/63478-v • 09:36 min • March 23rd, 2022
March 23rd, 2022 •
1, 2,3, 2,3, 1,2,3,4, 2,4,5
1Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Laboratorio de Imagen Médica, Instituto de Investigación Sanitaria Gregorio Marañón, 3Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 4CIBER de Salud Mental (CIBERSAM), 5High Performance Research Group in Physiopathology and Pharmacology of the Digestive System (NeuGut), Universidad Rey Juan Carlos

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We describe a preclinical experimental method to evaluate metabolic neuromodulation induced by acute deep brain stimulation with in vivo FDG-PET. This manuscript includes all experimental steps, from stereotaxic surgery to the application of the stimulation treatment and the acquisition, processing, and analysis of PET images.

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Keywords: Positron Emission Tomography Deep Brain Stimulation In Vivo Brain Dynamics Neural Networks CT Imaging MRI Stereotactic Frame Medial Prefrontal Cortex Surgical Procedure
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