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High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
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Biologia
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JoVE Journal Biologia
High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry

DOI:
10.3791/63485-v

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10:08 min

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July 06, 2022

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, , , , ,
1Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, 2Department of Plant Biology, Morrill Hall,University of Illinois at Urbana-Champaign, 3Environmental Plant Physiology group, Department of Plant Sciences,University of Cambridge

Capítulos

  • 00:04Introduction
  • 00:52Collecting Leaf Samples from Field
  • 02:11Preparing Samples for Analysis
  • 04:07Measuring of Non-Photochemical Quenching Using Chlorophyll Fluorescence Imager
  • 08:45Results: High-Throughput Method for Measuring the Relaxation of NPQ in Field-Grown Soybeans
  • 09:27Conclusion

Summary

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The protocol introduces a high-throughput method for measuring the relaxation of non-photochemical quenching by pulse amplitude modulated chlorophyll fluorometry. The method is applied to field-grown Glycine max and can be adapted to other species to screen for genetic diversity or breeding populations.

Tags

Keywords: High-throughput AnalysisNon-photochemical QuenchingPulse Amplitude Modulated Chlorophyll FluorometryGenetic DiversitySoybeanLeaf Discs24-well Plate96-well PlateNasal Aspirator FilterHumidity

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