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使用脉冲幅度调制叶绿素荧光法对作物中的非光化学猝灭进行高通量分析

JoVE Journal
Biologia

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JoVE Journal Biologia

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry

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使用脉冲幅度调制叶绿素荧光法对作物中的非光化学猝灭进行高通量分析

DOI:

10.3791/63485-v

•

10:08 min

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July 06, 2022

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Dhananjay Gotarkar², Lynn Doran², Meghan Burns², Abigail Hinkle, Johannes Kromdijk³, Steven J. Burgess²

¹Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, ²Department of Plant Biology, Morrill Hall,University of Illinois at Urbana-Champaign, ³Environmental Plant Physiology group, Department of Plant Sciences,University of Cambridge

Capítulos

00:04Introduction
00:52Collecting Leaf Samples from Field
02:11Preparing Samples for Analysis
04:07Measuring of Non-Photochemical Quenching Using Chlorophyll Fluorescence Imager
08:45Results: High-Throughput Method for Measuring the Relaxation of NPQ in Field-Grown Soybeans
09:27Conclusion

Summary

Tadução automática

该协议引入了一种高通量方法，用于通过脉冲幅度调制的叶绿素荧光测定法测量非光化学淬灭的松弛。该方法适用于田间生长的 甘氨酸max ，并且可以适应其他物种以筛选遗传多样性或繁殖种群。

Tags

High-throughput Analysis Non-photochemical Quenching Pulse Amplitude Modulated Chlorophyll Fluorometry Genetic Diversity Soybean Leaf Discs 24-well Plate 96-well Plate Nasal Aspirator Filter Humidity

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