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A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
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JoVE Journal Medicina
A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

DOI:
10.3791/63504-v

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06:45 min

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February 07, 2022

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, , , ,
1Department of Urological Oncology Surgery,Chongqing University Cancer Hospital, 2School of Medicine,Chongqing University, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science,Army Medical University

Capítulos

  • 00:05Introduction
  • 00:57Recipient Procedure
  • 03:04Donor Procedure
  • 04:44Implantation
  • 05:43Results: Cervical Heterotopic Heart Transplantation in Mouse Model
  • 06:08Conclusion

Summary

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In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.

Tags

Mouse Heart TransplantationCervical HeterotopicCuff TechniqueVessel AnastomosisCardiac AllograftImmunologic RejectionSurgical ProcedureRecipient MouseExternal Jugular VeinCommon Carotid ArteryMicrovascular ClampPolyurethane CuffSutureHeparin Saline

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