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Real-time detectie en vastlegging van invasieve celsubpopulaties uit coculturen
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
DOI:

08:00 min

March 30, 2022

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Capítulos

  • 00:05Introduction
  • 00:49Cell Culture
  • 01:17Cell Seeding and Assembly
  • 04:09Impedance Measurement
  • 04:44Detachment and Cell Collection
  • 05:54Results: Detection and Capture of Invasive Cell Subpopulations in Real-Time
  • 07:34Conclusion

Summary

Tadução automática

We beschrijven een aanpak om invasieve celsubpopulaties in realtime te detecteren en vast te leggen. Het experimentele ontwerp maakt gebruik van Real-Time Cellular Analysis door veranderingen in de elektrische impedantie van cellen te monitoren. Invasieve kanker, immuun-, endotheel- of stromale cellen in complexe weefsels kunnen worden vastgelegd en de impact van coculturen kan worden beoordeeld.

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