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Targeted Laser Ablation in the Embryo of Saccharina latissima

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Targeted Laser Ablation in the Embryo of Saccharina latissima

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Targeted Laser Ablation in the Embryo of Saccharina latissima

DOI:

10.3791/63518-v

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06:30 min

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March 11, 2022

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Samuel Boscq, Stéphanie Dutertre, Ioannis Theodorou, Bénédicte Charrier

¹UMR8227, CNRS / Sorbonne University, ²Univ Rennes, CNRS, Inserm, Biosit UAR 3480 US_S 018, MRic Core Facility

Capítulos

00:04Introduction
00:47Image Acquisition for Selecting Embryos for Ablation and Monitoring Subsequent Growth
01:31Laser Calibration
02:48Laser Ablation
04:21Monitoring the Growth of Irradiated Embryos
04:49Results: Effect of Local Cellular Laser Ablation on the Saccharina latissima Embryo
05:46Conclusion

Summary

Tadução automática

The destruction of specific cells in the embryo is a powerful tool for studying cellular interactions involved in cell fate. The present protocol describes techniques for the laser ablation of targeted cells in the early embryo of the brown alga Saccharina latissima.

Targeted Laser Ablation in the Embryo of Saccharina latissima

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