Journal
/
Bioengenharia
/
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
JoVE Journal
Bioengenharia
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioengenharia
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

DOI:
10.3791/63523-v

•

14:12 min

•

November 21, 2023

•

, , , , , ,
1Shanghai Key Lab of Modern Optical System,University of Shanghai for Science and Technology, 2Graduate School of Engineering,Osaka University

Capítulos

  • 00:09Introduction
  • 02:28Fabrication of CF-PCR Microfluidic Chip
  • 04:58Preparation of the PCR Solution
  • 05:36Construction of the CF-PCR System
  • 07:03Construction of the CE System
  • 08:34Run the PCR Solution
  • 09:48Detection of the PCR Products by this CE System Built Inhouse
  • 11:15Representative Results
  • 12:53Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes how to build a continuous-flow-polymerase chain system based on a microfluidic chip and how to build a capillary electrophoresis system in the lab. It presents a simple method for the analysis of nucleic acids in the lab.

Tags

Continuous-flow PCRMicrofluidic ChipCapillary ElectrophoresisNucleic Acid AmplificationEscherichia ColiPhotolithographySU-8 PhotoresistSoft BakeExposurePost-exposure Bake

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Fluorescence detection methods for microfluidic droplet platforms

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

A Microfluidic Chip for ICPMS Sample Introduction

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Read Article