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Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
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Capítulos
- 00:05Introduction
- 01:00Murine Allergic Rhinitis Model Establishment
- 02:03Isolation of Mononuclear Cells from the Nasal Mucosa
- 04:52Surface Staining for Flow Cytometry Analysis
- 06:08Results: Ovalbumin-Induced Murine Model Development to Explore the Role of ILC2s in Allergic Rhinitis
- 07:27Conclusion
Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.
