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Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models
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JoVE Journal Medicina
Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

DOI:
10.3791/63532-v

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06:16 min

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March 16, 2022

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, ,
1Department of Ophthalmology,University of North Carolina at Chapel Hill, 2Gene Therapy Center,University of North Carolina at Chapel Hill, 3Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill

Capítulos

  • 00:05Introduction
  • 01:26Preparation and Administration of Adeno-Associated Virus via Subconjunctival Injection
  • 04:21Results: Analysis of AAV Vector Administration, Biodistribution and Transgene Expression into the Subconjunctival Space
  • 05:41Conclusion

Summary

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In this manuscript, subconjunctival injection is demonstrated as a valid vector delivery method for ocular tissues in mice using an injection system consisting of an infusion/withdrawal syringe pump and a gastight removable syringe coupled with microinjection needles. This injection system is also adaptable for other intraocular administration routes.

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Keywords: Subconjunctival AdministrationAdeno-associated Virus VectorsGene TherapyMinimally InvasiveVisual ImpairmentBlindness36-gauge NeedleSterile Water70% AlcoholParafilmHamilton SyringeSyringe Pump

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