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Engineering Antiviral Agents via Surface Plasmon Resonance
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JoVE Journal Bioengenharia
Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

DOI:
10.3791/63541-v

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13:00 min

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June 14, 2022

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1Department of Environmental Health Sciences,University of California, Los Angeles, 2Department of General Surgery,Medical University of Vienna

Capítulos

  • 00:05Introduction
  • 01:05Preparation of LB-Agar Plates with Plasmid DNA Transformed Cells and Site-Directed Mutagenesis
  • 01:52Transformation of Bacterial Cells
  • 03:12Expression and Protein Purification
  • 06:22SPR Spectroscopy
  • 07:17SPR Binding Assay for Cyanovirin-N Binding to Hemagglutinin, S Protein, and RBD
  • 09:56Results: SPR Binding Assays to Examine Cyanovirin-N Binding to Hemagglutinin and Mannosylated Peptides
  • 11:55Conclusion

Summary

Tadução automática

Tadução automática

The present protocol describes new tools for SPR binding assays to examine CV-N binding to HA, S glycoprotein, related hybrid-type glycans, and high-mannose oligosaccharides. SPR is used to determine the KD for binding either dimeric or monomeric CV-N to these glycans.

Tags

Surface Plasmon ResonanceSPRProtein-ligand InteractionsVirus BindingKinetic AnalysisDrug DevelopmentAntibody CharacterizationDNA TemplateDpnI DigestionCompetent CellsTransformationSeed CultureExpression Culture

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