Journal
/
Biologia
/
Isolation of Primary Mouse Retinal Pigmented Epithelium Cells
JoVE Journal
Biologia
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Isolation of Primary Mouse Retinal Pigmented Epithelium Cells

Isolation of Primary Mouse Retinal Pigmented Epithelium Cells

DOI:
10.3791/63543-v

•

06:21 min

•

November 04, 2022

•

, , ,
1Eye Research Institute,Oakland University, 2Eye Research Center (OUWB)/ERC,William Beaumont School of Medicine, 3Oral Biology and Diagnostic Sciences, Dental College of Georgia,Augusta University

Capítulos

  • 00:01Introduction
  • 00:39Enucleation
  • 01:47Dissection
  • 04:43Centrifugation
  • 05:00Cell Culture
  • 05:15Representative Results
  • 05:58Conclusion

Summary

Tadução automática

Tadução automática

This manuscript describes a simplified protocol for the isolation of retinal pigmented epithelium (RPE) cells from mouse eyes in a stepwise manner. The protocol includes the enucleation and dissection of mouse eyes, followed by the isolation, seeding, and culturing of RPE cells.

Tags

Primary Mouse Retinal Pigmented Epithelium CellsRetinal Pigmented EpitheliumOuter Blood-retinal BarrierAge-related Macular DegenerationPovidone IodineHanks’ Balanced Saline SolutionRPE Cell Growth MediaEnzyme AEnzyme BOra SerrataDissectionIsolation

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Isolation of Retinal Stem Cells from the Mouse Eye

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Isolation of Immune Cells from Primary Tumors

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Primary Culture of Porcine Retinal Pigment Epithelial Cells

Read Article