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使用拉曼染料进行高度多重组织成像

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Highly-Multiplexed Tissue Imaging with Raman Dyes

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使用拉曼染料进行高度多重组织成像

DOI:

10.3791/63547-v

•

07:18 min

•

April 21, 2022

•

Lixue Shi, Mian Wei, Wei Min²

¹Department of Chemistry,Columbia University, ²Kavli Institute for Brain Science,Columbia University

Capítulos

00:04Introduction
00:59Preparation of Raman-Dye-Conjugated Antibodies
02:57Tissue Immuno-eprSRS Staining
05:02Image Acquisition and Analysis
05:35Results: Electronic Pre-Resonance Stimulated Raman Scattering Imaging of Raman Dyes
06:49Conclusion

Summary

Tadução automática

彩虹状拉曼染料的电子预共振受激拉曼散射（epr-SRS）成像是高度多重表位蛋白质成像的新平台。在这里，我们提供了一个实用指南，包括抗体制备，组织样品染色，SRS显微镜组装和epr-SRS组织成像。

Tags

Keywords: Highly-multiplexed Vibrational Imaging Protein Markers Subcellular Resolution Cycle-free Multiplexity Cell Characterization Tumor Microenvironments Brain Circuit Sodium Bicarbonate PBS Buffer N-hydroxysuccinimide Ester MARS Probe Antibody Conjugation Size Exclusion Column Purification

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