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1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences,University of South Florida, 2Cepheid (Danaher Corp.),US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment,Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences,De Montfort University
Capítulos
- 00:05Introduction
- 01:10Cell Lysis
- 02:56Electrophoresis
- 04:42Propidium Iodide Staining
- 05:43Results: Analyzing DNA Damage Using High Throughput Alkaline Comet Assay
- 07:17Conclusion
Kometanalysen er et populært middel for å oppdage DNA-skade. Denne studien beskriver en tilnærming til å kjøre lysbilder i representative varianter av kometanalysen. Denne tilnærmingen økte antall prøver betydelig samtidig som den reduserte analysens kjøretid, antall lysbildemanipulasjoner og risikoen for skade på geler.
