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通过非侵入性经鼻途径在免疫功能正常的小鼠大脑中移植人诱导的多能干细胞衍生小胶质细胞

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

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通过非侵入性经鼻途径在免疫功能正常的小鼠大脑中移植人诱导的多能干细胞衍生小胶质细胞

DOI:

10.3791/63574-v

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05:35 min

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May 31, 2022

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Bijay Parajuli², Youichi Shinozaki², Eiji Shigetomi², Schuichi Koizumi²

¹Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine,University of Yamanashi, ²GLIA Center,University of Yamanashi

Capítulos

00:04Introduciton
01:04Preparation of iPSMG
02:01Transnasal Transplantation
03:43Results: Viability of iPSMG in the Cortex and the Hippocampus
04:27Conclusion

Summary

Tadução automática

这里介绍的方案允许在免疫功能正常的小鼠中通过经鼻途径将诱导多能干细胞衍生的人小胶质细胞（iPSMG）移植到大脑中。图中显示了用于制备和经鼻移植细胞以及施用细胞因子混合物以维持iPSMG的方法。

Tags

Keywords: Induced Pluripotent Stem Cell-derived Microglia Transnasal Transplantation Immunocompetent Mice CSF1 Receptor Antagonist PLX5622 Non-invasive Neurodegenerative Diseases Hyaluronidase Cell Transplantation

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