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Transplantation af humant induceret pluripotent stamcelleafledt mikroglia i immunkompetente mushjerne via ikke-invasiv transnasal vej
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route
DOI:

05:35 min

May 31, 2022

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Capítulos

  • 00:04Introduciton
  • 01:04Preparation of iPSMG
  • 02:01Transnasal Transplantation
  • 03:43Results: Viability of iPSMG in the Cortex and the Hippocampus
  • 04:27Conclusion

Summary

Tadução automática

Protokollen, der præsenteres her, tillader transplantation af induceret pluripotent stamcelleafledt human microglia (iPSMG) i hjernen via en transnasal vej i immunkompetente mus. Fremgangsmåden til fremstilling og transnasal transplantation af celler og administration af cytokinblanding til opretholdelse af iPSMG er vist.

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