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Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays
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Biologia
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JoVE Journal Biologia
Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays

Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays

DOI:
10.3791/63599-v

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08:28 min

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August 26, 2022

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, , ,
1Department of Microbiology, Faculty of Agriculture,University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Capítulos

  • 00:05Introduction
  • 00:48Root Processing, Clearing, and Staining for Microscopy
  • 02:10Root Processing for Microscopy
  • 02:56Microscopic Analysis of the Root Samples
  • 03:26Post-Microscopy Image Assemblage and Scoring of Mycorrhizal Colonization
  • 05:05Raw Data Analysis and Result Extraction
  • 06:45Results: Assessment of the Arbuscular Mycorrhizal Colonization Patterns
  • 07:53Conclusion

Summary

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The protocol here describes the methods for the assessment of the arbuscular mycorrhizal colonization patterns and strategy in roots for two species: Zea mays and Festuca rubra. The use of the MycoPatt method permits the calculation of parameters, the conversion of mycorrhizal structures into digital data, and the mapping of their real position in roots.

Tags

Keywords: Mycorrhizal MapsArbuscular Mycorrhizal ColonizationRoot ClearingRoot RinsingRoot StainingRoot SegmentationMicroscopic AnalysisFestuca RubraZea MaysMycoPatt Method

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