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山羊窒息关节髌下脂肪垫间充质干细胞的分离、扩增和分化
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JoVE Journal Bioengenharia
Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint
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山羊窒息关节髌下脂肪垫间充质干细胞的分离、扩增和分化

DOI:
10.3791/63617-v

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12:06 min

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August 02, 2022

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, , ,
1Department of Biological Sciences and Bioengineering,Indian Institute of Technology-Kanpur, 2The Mehta Family Centre for Engineering in Medicine,Indian Institute of Technology-Kanpur, 3Mechanobiology Institute,National University of Singapore

Capítulos

  • 00:05Introduction
  • 01:08Isolation of Infrapatellar Fat Pad Mesenchymal Stem Cells (IFP-MSCs) from the Femorotibial Joint of Goat Knee
  • 05:16Maintenance and Expansion of Isolated Cells
  • 07:13Differentiation Potential of IFP-MSCs
  • 08:49Results: Expansion, Clonogenic Ability, and Differentiation Potential of IFP-MSCs
  • 11:07Conclusion

Summary

Tadução automática

Tadução automática

髌下脂肪垫间充质干细胞（IFP-MSCs）可以很容易地从膝关节的髌下脂肪垫中分离出来。它们 在体外增殖良好，形成CFU-F集落，并分化为成脂、软骨和成骨谱系。本文提供了从山羊扼杀关节分离、扩增和分化IFP-MSCs的方法。

Tags

Mesenchymal Stem CellsInfrapatellar Fat PadGoat Stifle JointIsolationExpansionDifferentiationRegenerative MedicineCartilage DefectsOsteoarthritis

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