É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Vocational School of Health Services/İstanbul,Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering,Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology,Istinye University, 43D Bioprinting Design & Prototyping R&D Center,Istinye University, 5Stem Cell and Tissue Engineering R&D Center,Istinye University, 6Medicine Faculty,Istinye University, 7Vocational School of Health Services,Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell),Liv Hospital
Capítulos
- 00:04Introduction
- 00:56Culturing and Cryopreservation of Wharton’s Jelly Mesenchymal Stem Cells
- 01:30Production of Exosomes from Wharton’s Jelly Mesenchymal Stem Cells
- 02:20Dopamine Loading into Exosomes and Characterization of Dopamine Loaded Exosomes
- 03:15Results: Development of a Nanocarrier System by Encapsulation of Dopamine into Exosomes Isolated from WJ‐MSCs
- 05:07Conclusion
Here, we aimed to obtain a formulation by dopamine loading of the isolated exosomes of stem cells from Wharton's jelly mesenchymal stem cells. Exosome isolation and characterization, drug loading into the resulting exosomes, and the cytotoxic activity of the developed formulation are described in this protocol.
