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基于FDR对照的非靶向代谢组的鉴定和定量集成工作流程

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

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基于FDR对照的非靶向代谢组的鉴定和定量集成工作流程

DOI:

10.3791/63625-v

•

05:35 min

•

September 20, 2022

•

Dehua Li*¹, Junze Liang*¹, Yongjian Zhang, Gong Zhang²

¹Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology,Jinan University, ²Chi-Biotech Co. Ltd.

Capítulos

00:05Introduction
01:07Preparing Metabolomics Datasets
02:21Metabolite Identification and Differential Analysis
03:49Results: Quantitative Analysis by MetaX
04:52Conclusion

Summary

Tadução automática

我们构建了一个非靶向代谢组学工作流程，将XY-Meta和metaX集成在一起。在该协议中，我们展示了如何使用XY-Meta从开放获取光谱参考中生成诱饵光谱库，然后进行FDR控制，并在鉴定代谢组学光谱后使用metaX定量代谢物。

Tags

FDR Control Untargeted Metabolome XY-Meta Target-decoy Strategy Metabolite Identification False Positive Reduction Biomarker Discovery GNPS Database ProteoWizard Raw Data Conversion NIST Library XY-Meta Program MetaX Software Quantitative Analysis PCA Plot Peak Annotation

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