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High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

DOI:
10.3791/63649-v

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09:31 min

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April 28, 2022

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, , , ,
1Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering,Hong Kong University of Science and Technology

Capítulos

  • 00:04Introduction
  • 00:55Open-Top Ultraviolet Photoacoustic Microscopy (UV-PAM) System
  • 05:10Sample Preparation
  • 06:17Experimental Procedures
  • 07:57Results: Ultraviolet Photoacoustic Microscopy (UV-PAM) Imaging with Deep Learning-Based Virtual Staining
  • 08:41Conclusion

Summary

Tadução automática

Tadução automática

A high-speed and open-top ultraviolet photoacoustic microscope that can provide histological images intraoperatively for surgical margin analysis is demonstrated, including the system configuration, optical alignment, sample preparation, and experimental procedures.

Tags

Ultraviolet Photoacoustic MicroscopyHistological ImagingVirtual StainingDeep LearningIntraoperative Surgical MonitoringTumor ResectionPositive Surgical MarginQ-switch Diode-pumped Solid-state LaserGalvanometer MirrorUltrasound TransducerData AcquisitionSample HolderAlignment

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