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Genom-dækkende profilering af transkriptionsfaktor-DNA-bindende interaktioner i Candida albicans: En omfattende CUT &RUN-metode og dataanalysearbejdsgang
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Biologia
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JoVE Journal Biologia
Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow

Genom-dækkende profilering af transkriptionsfaktor-DNA-bindende interaktioner i Candida albicans: En omfattende CUT &RUN-metode og dataanalysearbejdsgang

DOI:
10.3791/63655-v

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07:48 min

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April 01, 2022

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, , , , , ,
1Department of Molecular and Cell Biology,University of California, Merced, 2Quantitative and Systems Biology Graduate Program,University of California, Merced, 3Department of Applied Mathematics,University of California, Merced, 4Health Sciences Research Institute,University of California, Merced

Capítulos

  • 00:04Introduction
  • 00:49Isolation of Nuclei and Primary Antibody Binding
  • 02:40Binding of pAG-MNase to Antibody
  • 03:36Library Preparation for Sequencing
  • 05:22CUT&RUN Sequence Analysis
  • 05:54Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
  • 07:22Conclusion

Summary

Tadução automática

Tadução automática

Denne protokol beskriver en eksperimentel metode og dataanalysearbejdsgang for spaltning under mål og frigivelse ved hjælp af nuklease (CUT & RUN) i det humane svampepatogen Candida albicans.

Tags

Keywords: Genome-wide ProfilingTranscription Factor-DNA BindingCandida AlbicansCUT&RUNChIP-chipChIP-seqEpitope TaggingNuclei IsolationFluorescence MicroscopyAntibody IncubationProtein AG-MNaseData Analysis Workflow

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