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High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
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Bioquímica
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JoVE Journal Bioquímica
High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

DOI:
10.3791/63664-v

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08:48 min

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April 28, 2022

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, , , , , , ,
1Institut für Angewandte Physik,Universität Tübingen, 2Institut Max von Laue – Paul Langevin (ILL), 3Institut de Biologie Structurale,Université Grenoble Alpes, 4Institut Européen de Chimie et Biologie, Bordeaux INP, Chimie et Biologie des Membranes et des Nanoobjets (CBMN),Université de Bordeaux

Capítulos

  • 00:04Introduction
  • 01:04Preparation of H2O‐Hydrated Powders of Perdeuterated Protein
  • 03:14Incoherent Neutron Scattering Experiment
  • 06:57Results: Dynamics of Proteins and Hydration Water
  • 07:57Conclusion

Summary

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Tadução automática

Neutron backscattering spectroscopy offers a nondestructive and label-free access to the ps-ns dynamics of proteins and their hydration water. The workflow is presented with two studies on amyloid proteins: on the time-resolved dynamics of lysozyme during aggregation and on the hydration water dynamics of tau upon fiber formation.

Tags

Keywords: Neutron BackscatteringNeutron SpectroscopyProtein DynamicsHydration WaterPicosecond-nanosecond TimescaleAlzheimer’sParkinson’sSample PreparationHydrogen-deuterium Exchange

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