Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Journal

Bioquímica

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

DOI:

10.3791/63668-v

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11:38 min

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April 05, 2022

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Sohee Choi, Jessica A. San Juan, Marie C. Heffern, Michael J. Stevenson

¹Department of Chemistry,University of San Francisco, ²Department of Chemistry,University of California, Davis

Capítulos

00:04Introduction
00:59Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition
02:44Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand
03:56Isothermal Titration Calorimetry
09:00Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins
10:48Conclusion

Summary

Tadução automática

This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

•

•

•

Capítulos

Summary

Tadução automática

Tags

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

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Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

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