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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
JoVE Journal
Química
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JoVE Journal Química
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
DOI:

09:46 min

April 28, 2022

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Capítulos

  • 00:05Introduction
  • 01:00Preparation of Samples for Hyperspectral CRS Imaging
  • 01:55Instrumental Setup for Hyperspectral CRS Imaging
  • 04:18Image Analysis and Data Processing
  • 06:51Results: Comparing the Resolution, Sensitivity, and Imaging Contrasts of SRS and CARS
  • 08:54Conclusion

Summary

Tadução automática

This paper directly compares the resolution, sensitivity, and imaging contrasts of stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) integrated into the same microscope platform. The results show that CARS has a better spatial resolution, SRS gives better contrasts and spectral resolution, and both methods have similar sensitivity.

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