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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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JoVE Journal Engenharia
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

DOI:
10.3791/63709-v

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09:57 min

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July 25, 2022

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, , , , , , , ,
1Dipartimento di Fisica,Politecnico di Milano, 2Institute for Photonics and Nanotechnologies (IFN-CNR)

Capítulos

  • 00:04Introduction
  • 01:17Sample Preparation
  • 02:22Optimizing Pump and Stokes Beams
  • 05:54Setting Up the Spectral Detection for SRS Imaging
  • 07:07Chemometrics of Hyperspectral SRS Data
  • 07:54Results: RIN Spectra and the Effect of Balanced Detection
  • 09:15Conclusion

Summary

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We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

Tags

Stimulated Raman Scattering MicroscopyBroadbandVibrational SpectrumHyperspectral ImagingLabel-freeNon-invasivePMMA MicrobeadsPolystyrene MicrobeadsDimethyl SulfoxideOlive OilOptical Parametric OscillatorPrism CompressorDispersion Compensation

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