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原位冷冻切片斑马鱼胚胎中的杂交结合免疫组织化学

JoVE Journal
Neurociência

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JoVE Journal Neurociência

In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos

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原位冷冻切片斑马鱼胚胎中的杂交结合免疫组织化学

DOI:

10.3791/63715-v

•

07:36 min

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March 03, 2022

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Jiaqi Wang*^1,2, Rui Chai*³, Xiaoxia Fang, Jiajia Gu, Wanqing Xu, Qi Chen, Gang Chen³, Shunxing Zhu, Yan Jin

¹School of Life Sciences,Nantong University, ²Medical School,Nantong University, ³Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration,Nantong University, ⁴Nantong Maternal and Child Health Care Hospital, ⁵Laboratory Animals Center,Nantong University

Capítulos

00:04Introduction
00:39In Situ Hybridization
04:19Embedding and Cryosectioning
05:15Immunostaining
06:25Results: In situ Hybridization and Immunohistochemistry of Zebrafish Embryonic Sections
07:13Conclusion

Summary

Tadução automática

该协议描述了如何通过结合斑马鱼胚胎切片的原位杂交和免疫组织化学来获取图像。在冷冻切片之前进行原位杂交，然后进行抗体染色。如果抗体缺乏，检测斑马鱼中两个基因的表达模式是有用的。

Tags

In Situ Hybridization Immunohistochemistry Cryosectioned Zebrafish Embryos MRNA Protein Analysis Fixation Dehydration Rehydration Proteinase K Digestion Pre-hybridization Hybridization Probe Washing SSCT SSC

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