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小规模提取 秀丽隐杆线虫 基因组DNA
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Biologia
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JoVE Journal Biologia
Small-Scale Extraction of Caenorhabditis elegans Genomic DNA
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小规模提取 秀丽隐杆线虫 基因组DNA

DOI:
10.3791/63716-v

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06:40 min

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June 07, 2022

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, ,
1Department of Biology,Texas Woman’s University

Capítulos

  • 00:04Introduction
  • 00:51Single-Worm DNA Extraction
  • 02:54DNA Extraction of a Few Individuals
  • 04:33Results: Successful Use of the Extracted Caenorhabditis elegans DNA as PCR Template
  • 05:44Conclusion

Summary

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Tadução automática

这里介绍的是使用市售组织试剂盒从一只或几只动物中直接和相对快速地分离 秀丽隐杆线虫 基因组DNA的描述。所得的gDNA制剂是PCR的合适模板。

Tags

Caenorhabditis ElegansGenomic DNA ExtractionSmall-scaleClassroomPesquisaPCRLysis ProgramExtraction SolutionNeutralization SolutionL4Adult HermaphroditePlatinum Wire PickCentrifugation

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