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High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae
JoVE Journal
Biologia
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JoVE Journal Biologia
High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae
DOI:

29:23 min

January 03, 2008

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Capítulos

  • 00:11Introduction
  • 04:44Odor dilution
  • 08:20Preparation of larvae for tracking
  • 10:38Preparation of plates for tracking
  • 12:20Creation of odor gradient
  • 17:07Tracking system
  • 17:28Behavioral assay
  • 20:24Single source assay
  • 22:31Test of smell in individual larvae
  • 23:33Multiple source assay
  • 27:34Future directions

Summary

Tadução automática

In this video article, we describe a new method allowing the construction of odorant gradients with stable and controllable geometries. We briefly illustrate how these gradients can be used to screen for olfactory defects (full and partial anosmia) and to study more subtle features of chemotaxis behavior.

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