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Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

DOI:

10.3791/63800-v

•

09:40 min

•

June 30, 2022

•

Ali Ahmadi, Mohammad Balood, Katiane Roversi, Maryam Ahmadi, Moutih Rafei, Sebastien Talbot

¹Department of Pharmacology and Physiology,Université de Montréal

Capítulos

00:05Introduction
00:47Natural Killer (NK) Cell Isolation, Culture, and Stimulation
02:25Dorsal Root Ganglia (DRG) Neuron Isolation and Culture
06:13Co-Culture and Flow Cytometry
07:58Results: Interplay Between Natural Killer Cells and Nociceptor Neurons
08:41Conclusion

Summary

Tadução automática

Nociceptor neurons and NK cells actively interact in an inflammatory context. A co-culture approach enables studying this interplay.

Tags

Keywords: Natural Killer Cells Nociceptor Neurons Co-culture Anti-tumor Function NK Cell Isolation Mouse Spleen RPMI 1640 Medium IL-2 IL-15 Laminin Lumbosacral Joint Sacrum Lumbar Spine Atlantooccipital Joint

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