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心血管疾病大型动物模型中心脏的解剖技术和组织学取样

JoVE Journal
Medicina
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JoVE Journal Medicina

Dissection Techniques and Histological Sampling of the Heart in Large Animal Models for Cardiovascular Diseases

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心血管疾病大型动物模型中心脏的解剖技术和组织学取样

DOI:

10.3791/63809-v

•

10:53 min

•

June 16, 2022

•

Irina Constantin, Alexandru Flaviu Tăbăran

¹Department of Pathology,University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Capítulos

00:04Introduction
00:59Necropsy Protocol
04:16Dissection Protocol
06:21Sampling Protocol for Histology
07:53Sampling of Coronary Arteries
08:40The Four Chambers/Echocardiographic Plane Dissection Technique of the Heart
09:46Results: Visualization and Histological Examination of the Heart
10:13Conclusion

Summary

Tadução automática

由于解剖结构复杂，心脏样本的一致可重复解剖和收集方案的实施可能具有挑战性。本手稿介绍了一些标准心脏夹层方案的关键要素，重点介绍了大体检查方法和通常用于组织病理学检查的采样部位。

Tags

Keywords: Dissection Techniques Histological Sampling Heart Large Animal Models Cardiovascular Diseases Heart Anatomy Necropsy External Examination Skin Incision Abdominal Wall Diaphragm Ribcage Pericardial Cavity Visual Presentation Methodology Observer Uniformity Reproducibility Experimental Research

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