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来自大脑的淀粉样原纤维核心的生化纯化和蛋白质组学表征

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

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来自大脑的淀粉样原纤维核心的生化纯化和蛋白质组学表征

DOI:

10.3791/63816-v

•

09:00 min

•

April 28, 2022

•

Arun Upadhyay, Robert J. Vassar, Jeffrey N. Savas

¹Department of Neurology,Northwestern University Feinberg School of Medicine

Capítulos

00:04Introduction
01:01Enrichment of SDS Insoluble Material
03:29Amyloid Purification
04:17Methanol Chloroform Precipitation
04:58Trypsin Digestion
06:02Peptide Cleanup
07:12Results: Confirmation and Validation of Amyloid Purification
08:14Conclusion

Summary

Tadução automática

这种基于质谱的蛋白质组学分析的生化纯化方法有助于淀粉样原纤维核心的稳健表征，这可以加速预防阿尔茨海默病的靶标的鉴定。

Tags

Amyloid Fibrils Amyloid Plaques Alzheimer’s Disease Protein Purification Proteomic Characterization Homogenization Sucrose Gradient Protein Aggregates Protein Deposits

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