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1Department of Development and Regeneration, Woman and Child Unit, CF research lab,KU Leuven, 2Department of Pediatrics, Pediatric Pulmonology,University Hospitals Leuven, 3VIB Bio Imaging Core,VIB-KU Leuven Center for Brain & Disease Research, 4Department for Neuroscience,KU Leuven, 5Interuniversity Center for Biostatistics and Statistical Bioinformatics,University of Leuven and University of Hasselt, 6Department of Pediatrics, 2nd Faculty of Medicine,Charles University and Motol University Hospital, 7Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID),KU Leuven, 8Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL),KU Leuven, 9Department of Gastroenterology and Hepatology,University Hospitals Leuven, KU Leuven, 10Department of Chronic Diseases, Metabolism and Ageing; Pneumology,KU Leuven, 11Department of Respiratory Diseases,University Hospitals Leuven
Capítulos
- 00:04Introduction
- 00:59Organoid Imaging Using Confocal Microscopy
- 02:49Image Analysis and Measuring the Indices in the Imaging Software
- 05:55Results: Morphological Characteristics Determination Using Rectal Organoid Morphology Analysis
- 07:13Conclusion
Dieses Protokoll beschreibt die rektale Organoidmorphologieanalyse (ROMA), einen neuartigen diagnostischen Assay für Mukoviszidose (CF). Morphologische Merkmale, nämlich die Rundheit (Zirkularitätsindex, CI) und das Vorhandensein eines Lumens (Intensitätsverhältnis, IR), sind ein Maß für die CFTR-Funktion. Die Analyse von 189 Probanden zeigte eine perfekte Unterscheidung zwischen CF und Nicht-CF.
