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An Integrated Approach for Microprotein Identification and Sequence Analysis
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Biologia
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JoVE Journal Biologia
An Integrated Approach for Microprotein Identification and Sequence Analysis

An Integrated Approach for Microprotein Identification and Sequence Analysis

DOI:
10.3791/63841-v

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09:37 min

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July 12, 2022

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, ,
1The Heart Institute, Division of Molecular Cardiovascular Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine

Capítulos

  • 00:04Introduction
  • 00:40Loading the Phylogenetic Codon Substitution Frequencies (PhyloCSF) Track Hub to the University of California Santa Cruz (UCSC) Genome Browser
  • 01:25Navigating to Genes of Interest Using Gene Identifiers
  • 02:20Navigating to Genomic Regions of Interest Using Sequence Information
  • 03:10Identifying Conserved Short Open Reading Frames (sORFs) Using PhyloCSF Track Data
  • 04:06Viewing Homologous Regions in Other Genomes
  • 04:51Generating Multi-Species Sequence Alignments for Microproteins of Interest
  • 06:47Results: Identification of Genomic Regions of Interest for Microprotein-Coding Potential
  • 08:47Conclusion

Summary

Tadução automática

Tadução automática

The protocol described here provides detailed instructions on how to analyze genomic regions of interest for microprotein-coding potential using PhyloCSF on the user-friendly UCSC Genome Browser. Additionally, several tools and resources are recommended to further investigate sequence characteristics of identified microproteins to gain insight into their putative functions.

Tags

Microprotein IdentificationSequence AnalysisPhyloCSFUCSC Genome BrowserNon-coding RegionsProtein-coding PotentialComparative GenomicsBioinformaticsBlatGenome AssemblyDNA SequenceProtein Sequence

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