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1Putuo People’s Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology,Tongji University, 2National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics,Chinese Academy of Sciences, 3Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital,Fudan University
Capítulos
- 00:04Introduction
- 00:44Preparation of Myelin Oligodendrocyte Glycoprotein Peptide (MOG35-55) Emulsion and Pertussis Toxin
- 01:55Establishment of Experimental Autoimmune Encephalomyelitis (EAE) Animal Model
- 02:55Open Field Test
- 03:42Analysis of Bone Phenotype
- 04:16Results: Induction of EAE by MOG35-55 Treatment
- 05:49Conclusion
Le présent protocole décrit l’induction d’une encéphalomyélite auto-immune expérimentale dans un modèle murin utilisant la glycoprotéine oligodendrocytaire de myéline et la surveillance du processus de la maladie à l’aide d’un système de notation clinique. Les symptômes expérimentaux liés à l’encéphalomyélite auto-immune sont analysés à l’aide d’une micro-tomodensitométrie du fémur de souris et d’un test en plein champ pour évaluer le processus de la maladie de manière exhaustive.
