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Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii
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Biologia
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JoVE Journal Biologia
Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

DOI:
10.3791/63869-v

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03:37 min

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May 06, 2022

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Noriko Ueki1, Atsuko Isu2, Ken‒ichi Wakabayashi2,3
1Science Research Center,Hosei University, 2Laboratory for Chemistry and Life Science, Institute of Innovative Research,Tokyo Institute of Technology, 3School of Life Science and Technology,Tokyo Institute of Technology

Capítulos

  • 00:04Introduction
  • 00:46Preparation of Demembranated Cell Models
  • 01:47Reactivation of Demembranated Cell Models
  • 02:16Results: Demembranation and Reactivation of Chlamydomonas reinhardtii Cell Models
  • 02:56Conclusion

Summary

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The in vitro reactivation of motile cells is a crucial experiment in understanding the mechanisms of cell motility. The protocol describes reactivating the demembranated cell models of Chlamydomonas reinhardtii, a model organism to study cilia/flagella.

Tags

ReactivationDemembranated Cell ModelsChlamydomonas ReinhardtiiCilia MotilityCalcium Ion ConcentrationsIn Vitro ObservationMotile Cilia ResearchCentrifugationWashing BufferDemembranation BufferCell PelletDilution BufferMicroscope ObservationReactivation Solution

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